Composite

Part:BBa_K4863003:Design

Designed by: Zhihan Emma Chen   Group: iGEM23_Keystone   (2023-10-10)


PpsbA2_SpyTag_Slp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal NheI site found at 1181
    Illegal NheI site found at 1278
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal BglII site found at 2693
    Illegal BamHI site found at 3599
    Illegal BamHI site found at 4913
    Illegal XhoI site found at 816
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2763
    Illegal AgeI site found at 3111
    Illegal AgeI site found at 3171
    Illegal AgeI site found at 4692
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible linker is used to fuse SpyTag to Slp.


Source

Synechocystis PCC 6803

References